Importantly, most recent studies reveal an increasing awareness of possible limitations and pitfalls of the metabolomics approach, together with efforts for improved study design and statistical validation, which are crucial requisites for the sound development of NMR metabolomics and its progress into the clinical setting.
And a problem statement is often crucial and requisite to any cogent advice.
A crucial pre-requisite for large-scale annotation of eukaryotic genomes is the definition of what constitutes a gene.
According to the "vascular hypothesis of lung development," endothelial cell signaling is a crucial pre-requisite for the formation of the alveolar structure [30].
The success on these items will have important repercussions on the trust-building process between developed and developing countries, which is a crucial pre-requisite for advancing in a global climate deal [33].
The safety of hydrogen infrastructure is one of the crucial pre-requisites for a sustainable economy and accordingly, its design should be made based upon the performance to investigate and evaluate the risks from or out of the required infrastructure.
Irrespective of host systems, adequate a priori design and engineering of the molecular construct supported by good biophysical understanding of the single chain fragment molecules, is a crucial pre-requisite for improved product stability and downstream recovery, which will favourably impact final product yield and functionality.
So, based on Emx2OS down-regulation in Emx2-null mutants as well as on consequences of OS1-179 overexpressinn in rhombo-spinal precursors, we can speculate now that mutual promotion of sense and antisense transcription at the Emx2 locus may be a crucial pre-requisite for proper activation and expression of Emx2.
One of the crucial pre-requisites of the NO-mediated sGC activation is the presence of the reduced haem moiety where its oxidation or loss renders the enzyme insensitive to NO. Nitric oxide-independent sGC activators have emerged as valuable tools to elucidate the physiopathology of the NO-sGC-cGMP signaling pathway [ 36].
