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Due to the high density of plating, at the end of the longer culture time we analyzed, cell culture dishes are crowded of cells so it appears not possible to prolong more the culture without detaching and replate cells.
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Then, matrigel containing preparations were allowed to solidify at 37°C and cells loading were formed a compact crowd of cells.
Answer to this question, in part, may be explained by the molecular crowding of cells including naturally occurring osmolytes ([15], [16]).
This cuisine draws some of the same trend-mongering adherents that modern yoga does, which means that the line to order is clogged with an international crowd of cell-phone-blabbing Prada-wearers.
Any reaction that depends on the available volume can be affected by macromolecular crowding, but the effects of crowding in cells are complex and difficult to track.
Taken together with our current finding for mammalian cells, these results suggest that the appearance of aerobic glycolysis provides an energetically favorable catabolic state for all rapidly proliferating cells due to the inherent physicochemical constraint of molecular crowding on cell metabolism.
One explanation for clustering of organelles in microsporidia-infected cells is crowding of organelles in the peripheral cytoplasm of the host as the PV enlarges.
One possible scenario is that the dual function of contact, which delays apoptosis of crowding tumor cells but also inhibits their proliferation combined together with contact suppression of homing genes, ultimately, directs cell spreading and tumor expansion toward less populated niche regions where the 'contactless' gene signature is supposed to be less suppressed.
Long lines of this Town's young, addicted and lost, were filing into already crowded jail cells.
This work represents a step toward high-throughput and reference-free Visual Proteomics analysis of highly crowded whole-cell cryo-electron tomograms.
The map of case study 2 is somewhat more crowded with a ratio of cells : samples of around 2 : 1 and as well will see in the section on 'Hit histograms', some cells are the BMUs of more than one sample.
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