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Selection operation is conducted by comparing the parent vector X j k + 1 with the crossover vector u j k + 1.
The crossover operation in DDE delivers the crossover vector u j k + 1 by mixing the components of the current vector X i and the above mutant vector V i.
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To investigate the importance of PfPV1 for blood stage parasites, we generated a knockout plasmid, based on the double-crossover vector pHTK [12].
In the binomial crossover, the target vector is mixed with the mutated vector, using the following scheme, to yield the trial vector (u_i^G ).
Operation vector crossover An improved precedence preserving order based on crossover (iPOX), inspired from Lee et al.
Perform crossover for each target vector ( {X}_i^g ) with its noisy vector ( {X}_i^{/ g} ) and create a trial vector ( {X}_i^{// g} ) such that {X}_i^{// g}=left{begin{array}{c}hfill {X}_i^{/ g}kern1em,kern1em mathrhoif}kern1em rho le {C}_Rhfill hfill hfill hfill {X}_i^gkern1em,kern0.5em mathrm{Otherwise}hfill end{array}right.kern2em,kern1em iin {mathrm{N}}_{mathrm{P}} (15).
Perform crossover for each target vector ( {X}_i^k ) with its noisy vector ( {X}_i^{/ k} ) and create a trial vector ( {X}_i^{// k} ) such that {X}_i^{// k}=left{begin{array}{llll}hfill & hfill & hfill & hfill {X}_i^{/ k},hfill & mathrm{if}hfill & rho le {C}_Rhfill & hfill hfill & hfill & hfill &, iin {N}_Phfill {X}_i^k,hfill & mathrm{otherwise}hfill & hfill & hfill end{array}right.
The Pal depletion strain (CJW3131) was obtained by single crossover of the suicide vector pXCC3229.
Without considering mutations, each chromosome in each descendant individual can be represented using a pair of vectors: a vector of crossover positions and a vector of founder origins.
An adaptive search operator based on a problem-specific crossover and a two-vector crossover helps to balance exploitation and exploration.
Changing the position of the target gene mutation relative to the DSB in the plasmid also altered the distribution of targeted insertion subclasses recovered in wild-type cells, consistent with two-ended strand invasion followed by resolution into crossover-type products and vector integration.
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