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The msh4 –R676W hypomorph shows genome-wide reduction in crossover number compared to wild type.
Four different sets of β-lactamase mutants were constructed for the estimation of the crossover number.
To estimate the crossover number, the cells were plated on LB-chloramphenicol-plates (20 μg/mL).
Interference limits crossover number and ensures that crossover events are widely spaced along the chromosome (Muller 1916; Hillers 2004; Kleckner et al. 2004; Stahl et al. 2004).
Additionally, the ability to control the crossover number via tunable parameters is desirable as it enables access to different areas of sequence space.
Crossover number in these genetic maps does not differ dramatically from that observed in the same genomic regions in mapping populations for Morex × Barke or Morex × Steptoe.
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Despite the sheer beauty of his sound, Clayton is not totally convincing in either the Britten or the crossover numbers.
Statistically significant reduction in crossover numbers was observed on all chromosomes in the msh4 mutants.
In comparison, DNA shuffling can produce higher crossover numbers, while the recombination-dependent PCR frequently results in one crossover.
The msh4 –R676W hypomorph showed on average ∼30% genome-wide reduction in crossover numbers and reduced interference compared to wild type.
In these chromosomes as well, crossover numbers were mostly maintained at 1.6 per chromosome although some exceptions were observed.
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