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Parent plants used in crossing to generate cycle 1 and cycle 2 were not pure lines but segregating S0-derived S1 selections, which generated segregating S0 progeny.
One pair of insects from each BPH strain was selected for crossing to generate the IBSs H66 for Hadano-66, C89 for Chikugo-89, and K10 for Koshi-10.
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Mice expressing single MHC class I alleles (homozygously) were crossed to generate F1 generations with the following genotypes: Kb+Dd, Db+Dd, Kb+Ld and Db+Ld [16].
The Chk2 +/− Terc +/− mice were further crossed to generate successive generations (G1 G3) of Chk2 −/− Terc −/− mice, which progressively shorten telomeres.
Finally, mice from the G2 generation were crossed to generate the eight-way progeny, known as the G2 F1.
Bmx-CreERT2 mice37 and conditional β-catenin constitutive-activated mice (Ctnnb1lox ex3)/lox(ex3) mice)38 were crossed to generate EC-specific, tamoxifen (TAM -inducible β-caTAM -inducibleed mice (Bmx/CA mice).
Finally utrophin heterozygous 3cv mice were crossed to generate uko/3cv mice (Figure 1B).
Male and female Ehd3+/– mice were crossed to generate Ehd3+/+, Ehd3+/– and Ehd3 /– mice.
Ogg1 null and Myh null mice were crossed to generate Myh+/− Ogg1+/− mice.
Myh null and Ogg1 null mice were originally crossed to generate Myh+/− Ogg1+/− mice [16], [19].
Double heterozygotes were then crossed to generate the Dnmt3a−/− or Dnmt2−/− homozygotes.
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