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Into their indigo tongues, four crosses were set, woven with brass beads.
Gal4>UAS crosses were set up simultaneously as the Gal4>UAS; RelE20 crosses.
Crosses were set up in the evening and the barrier was lifted in the next morning.
Crosses were set at 25°C and then either grown at 25°C or transferred to 29°C after 3 days of egg laying.
To analyze GFP-tagged reporter mRNA in somatic muscles of stage 16 embryos the following crosses were set up: 24B-Gal4 mands and UAS-GFP-MS2-nls females were crossed.
An H-female was mated to an L-male (P generation), and subsequently, 15 full-sib F1 crosses were set up by combining random brothers and sisters to produce segregating F2 offspring.
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The four white crosses are set in a row.
Ten replicate cages of each type of cross were set up (dl x dl, wt x dl, and wt x wt).
Criss Cross is set in Los Angeles, and many readers will find Ellroy's dark version of the city half familiar from cinema: the narrator talks as though we know the place.
"My eyes have never hung so low as these past few days," he said, his gaze landing on the spot where a four-foot wooden cross was set ablaze on Nov. 21, the night before Thanksgiving.
In one upstairs gallery a small, pious painting of Jesus' head, lightly bearing the weight of the cross, is set on a small table just to one side of a wild abduction scene, Titian's "Europa".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com