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Vesco reports that many of the female hybrids were sterile, so the crosses were obtained only via backcrosses with male hybrids (Vesco's photos are obtainable via Additional File 1).
Initially, F1 offspring of within- and between-population crosses were obtained using 5 to 9 maternal plants from each population.
Embryos from RhoE +/gt mice crosses were obtained by cervical dislocation or by overdose of pentobarbital of the mother at 14.5 days post coitum (dpc).
Thirty F1 hybrid families from seven different cross types and three families of each of the nine homospecific crosses were obtained (see Table 1 for number of families and number of individuals per cross type).
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Parents and progeny of the HB3 × Dd2 genetic cross were obtained from the original cloned stocks.
Four-week old pigs (Yorkshire cross) were obtained from the Washington State University College of Veterinary Medicine (Pullman, WA, USA).
Publically available ChIP-seq of H3K4me3 (GSM307605) and H3.3 (GSM487542) in mouse ESCs derived from a SV129 × Cast cross were obtained and aligned to the mouse genome.
SNP and other sequence variation annotation results among the parental strains within each cross were obtained using the open-source tools SnpEff and SnpSift version 3.3h (Cingolani et al. 2012).
The results show that the I V curve crossing is obtained in asymmetrical devices under certain conditions only.
Cross-references were obtained from the ChEBI ontology file.
Cross-sections were obtained and the coating thickness was measured.
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