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Successful crosses were identified based on luciferase imaging in the F1 generation and plants were allowed to self-pollinate.
Differentially expressed genes between the crosses were identified in GeneSpring using a number of statistical methods and criteria.
Genotypes at every non-duplicated polymorphic locus in the haploid crosses were identified during the creation of the reference database.
Crosses were identified according to the number of the female parental line (D. koepferae) preceding the number of the male parental (D. buzzatii) line (e.g. the cross between D. koepferae line 48 and D. buzzatii line 55 was designated as 4855).
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Single plants representing independent events from each of 47 familiesies from the experimental cross were identified, and analysed by PCR for crossover within Ga1.1 nga8 nga1111 intervals (Figure 1C).
Polymorphisms segregating in the WILD1 cross were identified by sequencing E24C3 PCR amplicons from the P1 and F2 founders.
The third, killed later in the day near the Erez crossing, was identified as a member of Hamas's paramilitary police, known as the Executive Force.
Because the 18-bp/4-bp polymorphism is in the intron of sfl, a SNP in the cDNA that could be used to distinguish the two alleles in each cross was identified.
With these stringent criteria, it would be unlikely that the cross-links were identified by random chance, and the remaining cross-links should be correctly assigned.
A total of 3199 keel cross-sections were identified during the 2007 and 2008 ice seasons using a new processing method.
Only two cases of proven cross-transmission were identified among the nine acquired ESBL carriers.
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