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Because WILD2 consisted of several crosses, tests for homogeneity (G heterogeneity (h)) among crosses and fit to a 1 1 sex ratio in the data pooled across crosses (Gpooled (p)), were performed in addition to tests for fit to a 1 1 sex ratio for individual crosses (G) (Sokal and Rohlf, 1995).
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All of the crosses tested for PD identified a common QTL on linkage group J.
No commonality was detected in the other QTLs across the crosses tested.
Open field test and hole-cross tests were carried out to evaluate locomotor activity of animals.
The neuropharmacological activity of both open field and hole cross tests significant (p < 0.001) decrease the number of (square and hole) crosses by mice compare to control group.
It is clear that the plant extract significantly decreased the locomotor activity of mice in open field and hole cross tests when compared to the control (p < 0.05).
The methanolic extract of C. diffusa decreased the frequency and amplitude of movements in the open field and hole cross tests in mice.
Success or failure of copulation was confirmed by more than two replicate crossing tests.
When the original strain did not release gametes but zoospores, these could not be used directly for crossing tests.
Moreover, the validation of sedation was carried out by measuring external signs through hole-cross tests.
Central stimulating activity was investigated by pentobarbitone induced sleeping time, open field, and hole cross tests.
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