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For our experiments we randomly selected seeds of the F2 families of the within- and between-population crosses from each of the populations and sowed the seeds in the greenhouse in February 2006.
For this reason we set up a small number of single-pair matings first, as most viable insertions can be identified by evaluating just a few crosses from each subset.
Our comp-1 strains do appear to have a slightly reduced overall mating efficiency, typically affecting 1 to 2 crosses from each experimental repeat, which usually comprises 20 or more total crosses.
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In order to create F1 crosses, gametes from each strain were cross fertilized and reared in laboratory incubators.
The primary difficulty in creating computers is making logical circuits without wires crossing; wires cannot cross separate from each other in a 2-d world.
For each crosses, from more than 120 F2 individuals, no homozygous double mutants were detected.
One complete testis cross-section from each animal was analyzed, and the percentage of seminiferous cord cross-sections that contained one or more MNGs was recorded.
Twenty cross-sections from each condyle were examined by confocal microscopy to measure smoothness and depth of tissue damage to the articular cartilage caused by each of the 3 instruments.
One cross section from each processed tissue section was examined on hematoxylin-eosin stain.
We generate 10, 100, 500, and 1000 Kbps of cross traffic from each of five PCs.
The DSPR F1-hybrids were constructed by crossing individuals from each sub-population in the DSPR [ 32].
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