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Otherwise, four endos /Df or Df/+; endos /+ females were crossed to three y w males to test their fertility.
They were crossed to three males that were heterozygous or homozygous for the mutation tested, with no rescue construct.
Each of 10 trans-heterozygote males was crossed to three virgin females of D. simulans w; e for seven days.
From each crossing, two male offspring with yellow body color over the TM6C balancer were further crossed to three y w; Ly/TM6C virgin females.
The fertility tests were done as described in Material and Methods: each male 2 P was crossed to three females w; e and the progeny were counted.
The possibility that SOD1 loss of function contributes to ALS pathogenesis has been investigated by analysing the double mutant progeny of Sod1−/− or of Ccs−/− mice crossed to three tg SOD1-ALS lines overexpressing the human mutations, G93A, G37R and G85R.
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Males were crossed to two CS females, whereas females were crossed to two CS males.
The old3-1 heterozygous plants were crossed to two additional accessions.
For each cross, 20 virgin females were crossed to five males.
Four F1 females were independently crossed to four F1 male siblings to generate four F2 families.
MinnGold was crossed to two soybean accessions, 'Archer' and 'Minsoy', to develop mapping populations.
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