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The UAS-dE2f1-dsRNA transgenes were then crossed to multiple tissue-specific Gal4 drivers and the resulting phenotypes were characterized.
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Southern blotting was performed as described [26] and these lines were crossed to obtain multiple knock-out mutants.
Injected G0 animals were crossed to wild-type in multiple batches to generate G1 lines with independent ZFN mutagenesis events.
PhyloDetect [ 37] is one of the few analysis methods that deals explicitly with the genomic similarity between taxonomically related organisms, and the consequent tendency of some probes to cross-hybridize to multiple organisms.
Alternatively, these probes may contain conserved motifs and therefore the probes on the microarray will cross-hybridize to multiple transcripts resulting in the higher signal intensities observed on the microarray for these elements.
Because probes often cross-hybridize to multiple related genome sequences, the numbers of "expected" and "detected" probes may greatly exceed the number of probes that were actually designed for a given target organism.
Results from a recent analysis [ 5] indicated that on the Affymetrix GeneChip U95A/Av2 array, 20,696 (10.5%) probes were nonspecific, which could cross-hybridize to multiple genes, and 18,363 (9.3%) probes missed the target transcript sequences.
B The graphical user interface for DynaXL, in which one residue can be cross-linked to multiple residues with different cross-linking reagents.
This study set out to investigate the suitability of nanoindentation to extend the analysis of the cross-section to multiple points for a more detailed insight into variations through the thickness of the coating.
We here confirm that constitutive overexpression of Inhibitor of Apoptosis (IAP) survivin is indispensable for survival of HER2-positive BC cells with intrinsic cross-resistance to multiple HER1/2 inhibitors.
Not all paradigms that produce antinociceptive tolerance produce cross tolerance to multiple opioid drugs [28] [31].
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