When big data is used to determine children's educational pathways and thereby their future, or influence teachers' careers in education, we have crossed the line of appropriate use of big data in education".
To construct trx-1; daf-c double mutants, trx-1 homozygous males were crossed to the appropriate daf-c mutant, and F1 cross progeny hermaphrodites were grown singly at 25°C.
For controls, w or UAS GFP NLS was crossed with the appropriate Gal4 drivers.
G0 adults were crossed to the appropriate screening stock, and F1 progeny were screened for the presence of both transgenes.
Two individual w + male progeny were selected from each insertion line and crossed to the appropriate balancer chromosome.
To examine torsinA-mediated rescue of SOD1 (WT or mutant), integrated lines of torsinA [pan-neuronal (snb-1 promoter) or intestinal] were crossed with the appropriate SOD1 lines, generating the following lines.
To generate undead cells, UAS-rpr UAS-p35 or UAS-hid UAS-p35 flies were crossed to the appropriate drivers (hh-Gal4, ap-Gal4 and Ci-Gal4 were a gift from G Morata).
However, the ratio of viable conidia transferred from each strain at the end of an AG was measurable because, after their transfer, germinating conidia could be isolated and crossed with the appropriate reference strains to determine their fertility phenotype and hence their parental origin.
"My mother-in-law also, they both felt it crossed the line of what was appropriate.
When do you cross the boundary from appropriate child-raising to negligence?
