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And there are a huge number of strange spacecraft, shaped like opened grape scissors, or silver platters, or crossed fish forks, that hurtle through the galaxy at speeds rivalling the speed of light.
We crossed fish from Et kita GalTA4,UAS:mCherry)hzm1 line [13] with fish from the Tg(UAS:eGFP-H-RAS_GV12 io6 lineGFP-H-RAS_GV12 io6 eGFP-H-RAS_GV12 io6ing oncogenic HRAS in a pattern driven by kita regulinery sequences show an altered pigment pattern and increased pigmentation already starting from 3dpf (figure 1a, b).
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Sadoglu crossed cave fish with surface fish.
For testing the former hypothesis, we crossed tr265/tr265 fish with Tg(cmlc2:nuDsRed), a transgenic fish line that labels nuclei of all terminally-differentiated cardiomyocytes fluorescently red, to distinguish cardiomyocytes from non-cardiomyocytes (Figure 3A–B').
Sticklebacks from two independently derived freshwater populations [Fishtrap Creek (FTC) and Bear Paw Lake BEPAA)] were crossed to fish from a single marine population (LITC).
To ascertain whether axons beneath the DA developed from motoneurons or other neurons, we observed embryos expressing GFP under the mnx1 promoter, which is activated in motoneurons, crossed with fish expressing mCherry under the control of the fli1a promoter, Tg(fli1a:myr-mcherry);(mnx1 gfp) ml2 (Flanagan-Steet et al., 2005).
To examine individual neuron morphologies, we crossed these fish with carriers of a highly variegated UAS:mGFP construct contained within the Brn3c Gal4, UAS:mGFP (BGUG) transgene (Table 3; GFP refers to green fluorescent protein and Brn3c to a member of the POU domain transcription factor family that is expressed in specific neurons).
F0 fish were crossed with WT fish to identify germ-line transformed animals, as determined by fin cut PCR with GFP primers or control agonist treatments.
To assess the Tp53-dependence of ARF, we first crossed hs:ARF fish with tp53 mutantmutant fish to generate hs:ARF fish that are homozygous for the tp53M214K allele (hs:ARF; tp53 M214K/M214K).
The adult EGFP+ fish was crossed with the WT fish.
Sox10 eGFP (and sometimes also TRPC2 Venus) fish were crossed with PhOTO-N fish.
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