In other words, crossing alignment are not allowed.
Crossings should be planned and set out to preserve vegetation, except on the actual crossing alignment.
However, due to cross-alignment of probes, we cannot unequivocally assign cDNAs from the duplicate copies to a specific locus.
The genomic regions where the GS genes were located were examined in detail by determination of the open reading frames (ORFs) upstream and downstream of the specific GS genes and cross-alignment of these adjacent regions between the gene pairs.
Ordinarily this could result in cross-alignment, i.e. wild type reads derived from a given barcode could potentially be mapped to an incorrect barcode closely neighbouring in address space.
Our simple implementation was to replicate the barcode four-fold ("munging") such that a one base difference became amplified to four, thus defeating cross-alignment by Maq with default parameters.
For these genes, there are indications of differential expression in non-brain tissues by RNA-seq, although we cannot unequivocally demonstrate brain expression due to cross-alignment of cDNAs to multiple loci (Table 1).
Now, in one of the most star-crossed alignments in the continually consolidating media universe, Mr. Robertson finds himself in business, if not quite in bed, with his old nemesis, the Walt Disney Company.
However, the aligner fails to find the proper alignment because it does not consider all possible alignments crossing splice junctions owing to the search complexity.
The existing alignment is crossed by two seasonal streams with associated bridges.
East of Kingsland, the highway followed most of its present alignment but crossed the parallel railroad several times.
