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In some applications the incident particles will cross the detection plane with large angles w.r.t. the normal to the layers.
The Ct value is representative of the number of qPCR cycles required for the dye-DNA conjugate to be sufficiently amplified for the fluorescence to cross the detection threshold.
The cycle threshold (Ct) values, defined as the number of cycles required for the fluorescent signal to cross the detection threshold, of FluA and the H7 and N9 genes, and RP gene of the novel H7N9 virus did not obviously differ between the multiplex assay and WHO-recommended protocol (Table 4).
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Gmpgip3 crossed the detection of threshold 6 and 13 cycles before Gmpgip7 and Gmpgip5 transcripts, respectively.
Since IgM antibodies do not readily cross the blood brain barrier, detection of WNV IgM in CSF is diagnostic of neuroinvasive disease.
Then, a kind of perylene derivative (PTC-Lys), was synthesized by covalently binding L-lysine to 3,4,9,10-perylenetetracarboxylic acid, which was further immobilized onto the G-CdTe by the π-π* stacking and cross-linked the detection thrombin aptamer (TBA II) to obtain the TBA II/PTC-Lys/G-CdTe signal probes.
The Cp represents the point in the amplification cycle where the amplification curve crosses the detection threshold.
As soon as the heart rate crosses the lowest programmed detection zone, further analysis is performed to determine whether therapy is required.
The genes that crossed the threshold of detection P value ⩽0.01 among all the samples and differential P value ⩽0.05 among the test samples were considered to be the differentially expressed genes.
We used BRB Array Tool (v4.2.1; http://linus.nci.nih.gov/BRB-ArrayTools.html) to identify the 18 308 genes that crossed the threshold of detection P-value ⩽0.05 among all the samples and to derive by class comparison analysis (silenced vs control cells) the list of 476 differentially expressed genes with P-value<0.01 and FDR <0.25.
Jones was supposed to wave the van through the crossing, but the detection was made before the van reached his position.
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