The specificity of assay was cross tested with DNA of cattle, buffalo, sheep, goat, pig, duck, guinea fowl, turkey and quail, where amplification was observed only in chicken without cross reactivity with red meat species.
In these cases additional cross-tests with other known large-genome species (i.e. Oriza sativa and Solanum lycopersicon, nuclei prepared as for Arabidopsis) were performed, to check for the consistency of the results.
The statistical analysis performed using one-way analysis of variance (ANOVA) followed by Dunnett's post hoc test as appropriate using SPSS 19 software whereas open field and hole cross tests analyzed by ANOVA with repeated measures.
In hole cross test, the animal treated with LM showed dose dependent reduction in the locomotor activity.
The cross-reactivity tests with EGFR ECD were performed under the same conditions, except that ELISA plates were coated with EGFR ECD.
These data suggest that both models performed well in the 5-fold cross-validation tests with the RBF kernel slightly outperforming the linear kernel model.
By running cross-validation tests with the previously mapped contigs, we investigated the effects of training set size, sample imbalance, and bin sizes of the feature distributions on the classifier.
The molecular characterization of PR alleles confirmed for the sexual strains the results obtained with standard crossing tests.
Probe specificity was validated by performing a cross-reactivity test with dot blot hybridization.
Genetic crosses testing female viability with mutant gene combinations had the progeny counted 8 9 days out from the first day of eclosion.
* all cross-tabulation χ2 tests with p < 0.001 ** Classified according to reference quintiles estimated for Pelotas.
