Indeed, RRGS_HYB could perform better than RRGS_PAR because it exploited the within-cross phenotypic variation by associating it with the within-cross segregation of marker alleles.
A third locus (LG x) is also predicted and, although based on only a few crosses, segregation analysis suggests it is unlinked to the LG6 locus (i.e., 9 7 ratio in the progeny of LNF14self; Table 1 and Table 2).
For each cross, locus segregation was tested for goodness-of-fit to expected Mendelian segregation ratios using chi-square tests (P ≤ 0.01 with Bonferonni correction).
For each cross, Mendelian segregation ratios were tested using chi‐square goodness‐of‐fit tests.
In an RT × N cross, alternate segregation moves T and T to one spindle pole and N and N to the other to produce eight parental-type ascospores (i.e., 4 RT + 4 N) that are viable and black.
When hybrid females and pearl males (P2 generation) were crossed severe segregation distortion was observed: 98% of the progeny were EGFP positive, rather than the expected 50%.
barbadense which offers a higher polymorphism rate than intraspecific crosses, and segregation for superior fiber quality characteristics.
Several S1 self progeny from selected S0 plants were used in crossing, and segregation among these S1 genotypes resulted in some poor parents.
- The progeny of these crosses following segregation away of the mutant allele, denoted epiRILS (epigenetic Recombinant Inbred Lines), were evaluated over several generations for stability and inheritance of the methylated alleles contributed by the wild type parent and their unmethylated counterparts provided by the hypomethylated mutant.
Cross 8 showed segregation for the putative lip colour locus, L. The offspring phenotype frequencies would be consistent with single locus, assuming that L is part of the supergene and treating lip colour phenotypes as either normal (N) or albolabiate lip (A); there was no deviation from expected Mendelian ratios.
