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The data, including measured discharge, river width, water stage, water depth, wet area, and cross section, were collected from the hydrological yearbooks of typical hydrological stations on the Songhua River and the Yangtze River from 1955 to 1987.
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Cross sections were collected on glass slides and placed on a warming tray (40°C, 3 h).
Transverse 6 µm thick cross sections were collected along the entire length of the muscle at 300 µm intervals with a cryostat (Leica CM 1850) and stained with haematoxylin/eosin solution, PAS or assayed for COX activity.
Transverse 5 µm-thick cross-sections were collected along the entire length of the muscle at 100 µm intervals with a cryostat (Leica CM 1850).
IR microspectroscopic images of skin cross-sections were collected with a PerkinElmer Spotlight 300 system (PerkinElmer Life and Analytical Sciences, Inc., Waltham, MA, USA).
Pictures of stained cross-sections were collected using a Leica DM5000B fluorescence microscope, a digital camera (F-View; Soft Imaging System), and analySIS® software (Soft Imaging System).
Serial cross-sections were collected on glass slides and stained for succinate dehydrogenase (SDH) activity to determine the oxidative capacity of the muscle fibres, as described previously (Boerio et al., 2008; Boerio et al., 2010).
Every 10th section was collected for sampling.
Every tenth 20 µm section was collected.
Sections were collected on a 0.5% formvar coated slot grid.
The sections were collected on 100 mesh copper grids.
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