Real-time integration of the plume cross section is performed using a set of standard and constant values for wind speed and plume height.
Elemental analysis of polymer membrane cross sections was performed using an energy dispersive x-ray spectrometer and analyzed with Aztec 2.1 software (Oxford Instruments, UK).
Z-stack images were taken every 0.3 μM and 3D cross sections were performed using Leica confocal analysis software.
TUNEL assays in tissues cross sections were performed using DeadEnd™ Fluorometric TUNEL System (Promega, Madison, WI) according to the manufacturer's protocol.
Image processing, including identification of fiber type in all fibers on the muscle cross-section, was performed using Adobe Photoshop® CS3.
Myelin staining of spinal cord cross-sections was performed using FluoroMyelin (Invitrogen) to measure the area of myelinated nerve fibers in the white matter.
The optimization of the cross-section is performed using the trust-region method (TRM) based on the failure modes of yielding strength, deflection limitation, local buckling, distortional buckling and lateral torsional buckling.
As a comparative control, this same analysis was performed using a fresh cross-section of a Zea mays leaf, a Kranz type C4 species that is known to have low PSII content in BS chloroplasts.
The microstructural analysis was performed using thin foils from the cross-sections of nitrided samples.
Transmission electron microscopy was performed using ultrathin sections (Fig. 2D).
The design of the neutron collimator was performed using MCNP4C and the ENDF/B-V cross-section library.
