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Evidence of cross mobilization of hobo by an endogenous Homer element emphasises the necessity to understand the endogenous transposons within a species.
Subsequently, they have expanded through cross mobilization with different, non-homologous, mariner elements already present in the bug genome.
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Within the hAT superfamily, cross-mobilization has been reported for the hobo element, which is able to mobilize the hermes transposons (Sundararajan et al. 1999).
Recent genome-wide analyses and cross-mobilization assays have greatly improved our knowledge on MITE proliferation, however, specific mechanisms for the origin and evolution of MITEs are still unclear.
Although recent genome-wide analyses and cross-mobilization assays have greatly improved our knowledge on MITE proliferation [8], [9], [11] [14], specific mechanisms for the origin and evolution of MITEs are still unclear.
If this is the case, it might allow for cross-mobilization of these MITEs by various kinds of piggyBac-like transposons (Fig. 3), since TIRs of piggyBac-like transposons we identified in the A. millepora genome also preserved this motif (Table 2).
However, if the TIRs are similar enough, cross-mobilization may occur between two lineages from the same subfamily.
Cross-mobilization is highly associated with the amplification of MITE families (Jiang et al. 2003; Torres et al. 2006; Yang et al. 2009).
Use of co-transposition analysis could provide direct evidence for MITE transposition and able to confirm cross-mobilization between MITEs and the related autonomous elements.
Our finding that OPI is relieved by reducing the affinity between the transposase and the transposon end provides a further explanation for the unexpected efficiency of this cross-mobilization in addition to their short length.
These results were reminiscent of miniature inverted-repeat TEs (MITEs) that were highly transposable because of transposases encoded by distantly related and self-restrained autonomous elements in rice, a mechanism known as cross-mobilization.
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