Sexual differentiation was promoted by culturing on synthetic crossing medium (SCM) plates (Westergaard and Mitchell 1947).
Vegetative cultures were maintained on Vogel's medium (Vogel 1956), and crosses were performed on synthetic crossing medium (SC) of Westergaard and Mitchell (1947).
Vogel's minimal medium (VM /1.5%sucrose/2%% agar was used for vegetative growth and synthetic crossing medium (SCM /1%sucrose/2%% agar for development of female sexual structures (Davis and de Serres 1970).
Vegetative growth and asexual development (conidiation) were analyzed using Vogel minimal medium (VM) (Vogel 1956), whereas sexual development was assessed using synthetic crossing medium (SCM) (Westergaard and Mitchell 1947).
For tissue collection during sexual development, FGSC 2489 was first grown on synthetic crossing medium (Westergaard and Mitchell 1947) covered with cellophane (Midsci, St . Louis MO) in 245 × 245 × 25-mm bioassay dishes (Thermo Scientific, Hvidovre, Denmark).
Cultures of N. intermedia for microarray analysis and EST sequencing were grown in 90-mm Petri dishes on solid synthetic crossing medium (SCM) [ 59] with 2% sucrose, covered by a layer of sterilized cellophane membrane, and in test tubes with Vogel's minimal medium [ 60] with 1.5% sucrose.
These include lack of a U7H6-G6H2′ U7H6-G6H2′, medium cross-peaks for G8H8-G6H1′ and G8H8-G6H2′, a medium cross-peaksfor G8H8-G6H1′ G8H8-G6H1′ in 5′G ande G8H8-G6H2′ G8H8-G6H2′pporting Information), and an extremediumeak or absent G8H8-U7H6 cross-peak.
The exhibition's curatorial team, headed by Teresa A. Carbone, makes exceptional use of cross-medium or crosscultural contrasts that put the mind in two places at once.
It also confirms that the persistence of painting is but one part of a larger phenomenon: the cross-medium, transcultural ubiquity of the pictorial.
When "Smash" premiered — before its narrative dysfunctions became apparent — it was suggested that some day, once the show caught on, the fake musical could become a real one, a cross-medium spin-off like none the world had ever seen.
