Virgin driver females were crossed with UAS-chn males and crosses maintained at 18°C to repress GAL4.
As already mentioned above, hardly any double homozygous flies could be obtained from crosses maintained at 25 °C.
Eggs from each cross were maintained in isolation using standard commercial rearing methodologies.
The parents and F1 individuals from this cross were maintained at Wuhan Botanical Garden of the Chinese Academy of Sciences.
N. vectensis adults (descendents of the CH2 and CH6 cross) were maintained and spawned as described in Fritzenwanker and Technau [ 63].
Framed by the ever-present mountains, fields of white crosses, carefully maintained by middle- and working-class families, stretched as far as I could see.
All flystocks and crosses were maintained at 25°C.
All crosses were maintained at 25aC except for the interaction with Gal4-rpr that was at 19°C.
Fly stocks and crosses were maintained according to standard Drosophila culture procedures and were housed in a 25 °C incubator under 12∶12 light:dark cycle.
Crosses were maintained at 25°C.
Crosses were maintained at 18°C in standard medium.
