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Oocytes and sperm were obtained by dissection of gonoducts, dechorionation was performed with 1.0% sodium thioglycolate (T0632, Sigma) and 0.05% actinase E (D4527, Sigma) (Mita-Miyazawa et al., 1985) and cross fertilization was performed in glass tubes.
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Although oilseed rape is autogamous to a high degree, cross fertilization is common (12 to 55%) [ 4, 5].
Back when one could accurately describe classical music as European, the cross-fertilization was mainly regional.
However, artificial cross-fertilization was successful in both species, making genetic isolation a weak argument for the observed allelic divergence (Stevens et al., 2012).
Increased ploidy levels of the endosperm were observed in 13 measurements altogether; in 11 of them, the expected ploidy level of endosperm after cross-fertilization was observed.
It is a digonic protandrous hermaphrodite that even though it has the ability to self-fertilize, cross-fertilization is the main reproductive strategy when conspecifics are close enough [ 2].
Brenner focused on C. elegans because of its short generation time, ease of cultivation, and an unusual reproductive mode in which the presence of self-fertilizing hermaphrodites that can nonetheless mate with males for cross-fertilization is very useful for genetic studies.
I think such cross-fertilization is vital.
The possibility of artistic cross-fertilization is another plus.
Methods of cross-fertilization are equally diverse in animals.
And his knack for cultural cross-fertilization is winning him champions among American and European critics.
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