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A specific mitochondrial protein marker was shown not to be present in the nucleus after curcumin treatment, indicating that the fractionation procedure was not confounded by cross contamination (Figure 8C).
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We performed DNA fingerprinting to verify that TiPS shared a genetic background with the starting donor T-cell population and to rule out cell line cross-contamination (Figure S1).
All of the parasites in the panel were genotyped by PCR using MSP1/MSP2 primers during the course of the experiments to prevent cross-contamination (Figure S1) and, in addition, the authenticity of the 3D7csa parasite line was confirmed by sequencing PCR products from the highly polymorphic Pfama1 and Pfvar2csa genes (data not shown).
When all sequences were included in a single phylogenetic analysis, sequences from each mother-infant pair formed a strong monophyletic group, indicating that maternal and infant sequences were epidemiologically linked and there was no evidence of cross-subject contamination (Figure 1).
The different fractions of the cells were analyzed in western blot and by using antibodies against nuclei specific Cyclin E (Figure S2) and cytoplasmic and membrane located Ezrin (Figure S3) any cross contamination of the cellular fractions could be excluded.
The volumes shown in Figure 6, rather than Figure 5, used for the addition of cells in the red portions of the 96-well antimicrobial assays as designed in Figure 1, would lessen the probability of cross contamination.
Recent studies have shown computer keyboards to be a major source of cross contamination infections.
Despite noble efforts by cooks, gluten can still appear in seemingly innocent items either by cross contamination or hidden ingredients.
788 sickened in 2000 in Wisconsin, USA from raw beef, cross contamination of other foods (Sizzler).
The pieces are held in a secure location and there is no cross contamination.
Result allows to discard any DNA contamination (Figure S2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com