To address the contributions of aberrant chromosome rearrangements to the deleterious effects of TRF2 deletion in vivo, we crossed conditional TRF2 +/∆ K5-Cre with TRF2 +/+ 53BP1 −/− mice (Ward et al., 2003), to obtain TRF2 ∆/∆ K5-Cre 53BP1 −/− mice.
In order to test whether Pax3 is functionally required for the initiation or progression of p53-deficient BSG in vivo, we crossed conditional Pax3 knockout mice [ 38] into our p53-deficient BSG model [ 35].
In the uterus, biallelic deletion of PTEN has been achieved by crossing conditional PTEN-KO mice with a mice expressing Cre under the progesterone receptor promoter (PR-Cre) (Daikoku et al., 2008).
Similarly, for our comparison group, we crossed conditional Fbxw7 fl(Δex5)/+ null animals with Vill- Cre mice to give Vill-Cre; Fbxw7 fl(Δex5)/+ (Fbxw7 +/−) animals and confirmed efficient Cre-mediated recombination as above.
To this end, we crossed conditional TRF2 flox/flox mice or TRF2 +/flox mice with K5-Cre mice that constitutively express the Cre recombinase under the control of the keratin 5 (K5) promoter from day 11.5 of embryonic development onwards (Ramirez et al., 1994), thereby generating TRF2 Δ/Δ K5-Cre mice.
We observed dramatic drops in all P values for various combinations of amino-acid positions in the cross-conditional analysis adjusting for each of the three amino-acid positions (Table 1).
We generated heterozygous mice (Pik3c3+/-) by crossing the conditional allele (Pik3c3flox/flox) with Meox-cre transgenic mice in which Cre is expressed in the germ line [34].
More specifically this implies that the window period is the mean observable threshold crossing time, conditional on assay progression (i.e. actually reaching the threshold).
In this approach, a continuous latent factor with test- and class-specific effects is added to the 2LC model, supposedly to account for any effect which may contribute to the above cross-method conditional dependencies.
Orthology detection methods all tend to rely on a similar set of concepts for identifying protein pairs across species boundaries (Table 1), making cross-method conditional dependence a potential complication (Figure 3).
In order to circumvent this early embryonic lethality and to be able to probe the function of NMD during the development of a defined organ, we crossed the conditional Upf2fl/+ allele onto the Alfp-Cre strain thereby facilitating deletion of UPF2 during liver development.
