The finding "critically points out the need for understanding alien species," says ecologist Louis Gross of the University of Tennessee, Knoxville.
The authors offer some interesting thoughts on the reason for this null effect, most critically pointing to a need to have an active task PET design.
We thank the reviewers for critically pointing out that our statement 'autophagy passed through a lag phase ' was mis-leading, especially since we never intended to argue that the MVB pathway and autophagy are sequentially activated.
Samples in ethanol were then critically point dried using CO2 (Sorvall Critical Point Drying System).
Samples were spotted onto filter paper, critically point dried in an Autosamdri-815 critical point dryer, and coated with palladium gold.
For scanning electron microscopy (SEM), the inserts were removed from the wells, critically point dried on a Balzers Union critical point dryer with carbon dioxide as the exchange medium, and mounted on stubs using double-sided adhesive tape.
Subsequently the hydrogels were critically point dried in a Samdri pvt-3 critical point dryer (Tousimis, Rockville, MD).
Cells were dehydrated in a graded series of ethanol and critically point-dried with liquid CO2 in a BAL-TEC-CPD 030 Critical-Point Dryer (Bal-Tec AG, Balzers, Liechtenstein), mounted on aluminum stubs with silver paint (EM Sciences) and coated with gold in a BAL-TEC SCD 050 Sputter Coater (BAL-TEC).
Specimens were then critically point dried and coated with gold using sputter coating for examination using a scanning electron microscope (SEM), JEOL, JSM 5300.
Samples were critically point dried in a CO2 atmosphere (BAL-TEC CPD 030, Leica Microsystems GmbH, Wetzlar, Germany), sputter-coated with gold palladium by Hitachi ion sputter E-10455, HiTokyo, Japanokyo, Japan), then observed with a Hitachi SU8010 FESEM (Hitachi Co., Tokyo, Japan) at the Public technology service center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
