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A perfusion-based bioreactor system was critically employed to uniformly seed and culture hMSC in the scaffolds and to efficiently decellularize (94% DNA reduction) the resulting ECM while preserving its main organic and inorganic components.
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Then, the well-known DNA damage response in Saccharomyces cerevisiae was employed to critically evaluate whether these novel compounds can damage the DNA in vivo.
Further, because REDD+ remains a politically volatile issue, with both critics and supporters pointing to the impacts (or lack thereof) of these pre-REDD+ projects, it is important to critically examine the methods employed to assess those impacts.
Although the loop-diuretic furosemide is widely employed in critically ill patients with known long-term effects on plasma electrolytes, accurate data describing its acute effects on renal electrolyte handling and the generation of plasma electrolyte alterations are lacking.
It is noteworthy that certain pharmaceutical interventions commonly employed in critically ill patients can directly contribute to the development of hyperglycaemia.
In addition, a recent report cautions that systemic corticosteroid treatment should be very cautiously employed in critically ill patients in ICUs because of greatly increased risk of death due to bacterial pneumonias (Ranzani et al, 2012).
The comprehensive monitoring of cerebral physiology and biochemistry commonly employed in critically ill patients with acute brain injury (especially TBI and SAH) provides a valuable opportunity to challenge some of these assumptions in preliminary studies, or as part of early phase II trials, before proceeding to more definitive studies (Box 2).
Designers are in the difficult position of approaching these geographic datasets critically while simultaneously employing them in their work.
Concerns about the jury's ability to understand, critically evaluate, and employ scientific evidence in deciding complex trials have led to many suggestions for reform.
These effects need to be critically assessed when employing a newly developed labelling assay.
This paper discusses the main debates stimulated by the project and critically evaluates the methodology it employed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com