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The critical weight marks a developmental transition.
In Manduca this is the function of the critical weight.
To investigate whether the variation in carbohydrate concentrations was functional, we injected 5th instar larvae that were starved before they reached the critical weight with either glucose (1mg) or trehalose (0.5mg) before they had passed the critical weight.
Prior to the critical weight the transcript levels of Bbx in the brain and the InR in the wing disks declines when larvae are starved, but after the critical weight these transcript levels are unchanged after starvation.
Prior to the critical weight starvation leads to a rapid decline in the level of trehalose in the hemolymph, but after the critical weight starvation actually leads to a slight rise in trehalose.
In other pathologies (Alzheimer's disease, atherosclerosis, stroke, heart attack) a critical weight of deregulated population has to be reached.
When larvae were starved before they had attained their critical weight both body and wing disks stopped growing.
Thus after a larva passes the critical weight, growth of the wing imaginal disks becomes uncoupled from food intake.
Larvae were starved either before or after passing their critical weight and their hemolymph glucose and trehalose concentrations were measured.
Elevated JH titers could, therefore, explain the arrest in wing disk growth observed during starvation before the critical weight.
The peak weight of this strain is 11 12g, so the critical weight occurs about halfway during the growth phase.
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