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We have recently listed the critical technical points to amplify these low-abundance and GC rich mRNAs, and highlighted the need for a higher temperature and a gene-specific primer during the RT step [21] (see Materials and Methods).
Instead, it only applies to civilian employees and contractors of other federal agencies "to the extent such employment relates to supporting the mission of the Department of Defense overseas". This may seem like a technical point - but it's critical.
From the technical point of view, the density of the markers is also critical for the power of such studies and could be a source of discrepancy.
First, a technical point.
Critical Technical Practice.
One more technical point.
This is more than a technical point.
Now onto a more technical point.
So I agree with her technical point.
The critical technical assistance of Dr. Marina Savenkova is acknowledged.
The authors thank J.A. Lyons for critical technical assistance.
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CEO of Professional Science Editing for Scientists @ prosciediting.com