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An early warning criterion system which includes stress, water temperature and water pressure was established.
Undigested samples were prepared by mixing an aliquot of 10% brain homogenate with an equal volume of SDS loading buffer and were then boiled for 5 min. Thirty microliters of the PK-treated or undigested samples were loaded onto 12% SDS/PAGE Precast gels (Criterion system from BioRad).
The gels were run using the Bio-Rad Criterion system according to the manufacturer's instructions.
Proteins were separated on 10% SDS PAGE gels using the Criterion system (Bio-Rad, Hercules, CA) and transferred to nitrocellulose.
Renal cortex was prepared for Western blotting by rapidly dissecting frozen kidney tissue on ice, followed by homogenization using the Bio-Rad's Criterion system (Hercules, CA, USA), as previously described [ 25].
SDS-PAGE was done in a Criterion system (BioRad, Hercules, CA, USA) using stain-free gels (Criterion TGX Stain-Free Precast Gels, 4% to 20%) with 18 wells and Tris/glycine/SDS buffer as running buffer.
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No significant differences were seen in any metabolic variable between the two criterion systems (DBM and Oxycon Pro).
The definition of the developed model was dealt with, specifying the optimum quantity of economic ordering in the central and local warehouses; it designed and resolved a two-criterion system to minimize the related costs of inventory system and displacement systems.
BEAS as a multi-criteria system includes environmental, social and cultural aspects.
Successful supply chain management requires the management of a complex, multi-stakeholder, multi-criteria system.
Optimal criteria, system architecture, energy production, and cost analysis are discussed based on the characteristics of solar radiation exposure in South Korea.
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