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Cetuximab was used as a control to ensure criterion of tissue microarray.
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The criterion of tissue-specific expression is another routine computational filter [22] in target selection and is independent vs. non-mechanistic (marketing) factors.
The strict localization of integrins to basal cells underlining restored tissue polarity, the drop of keratinocyte growth rates towards physiological levels and the rapid formation of a mature basement membrane with abundant anchoring fibrils are altogether features fulfilling the criteria of tissue homeostasis.
By the criteria of tissue section IF, by expressing (or not) antigen in cells lacking antigen and by Western blotting all six antibodies were concluded to be nonspecific.
As detailed in the methods section, we based our criteria of tissue similarity on the lists of tissue-selective bimodal genes in common within each unique pair of tissues.
In contrast to the more catabolic state in conventional DEs, the de novo reconstruction of genuine dermal tissue seemed to be a key element for maintaining prolonged normal keratinocyte proliferation (followed up to 8 wks), fulfilling the criteria of tissue-homeostasis, and possibly providing a stem cell niche.
Several criteria of tissue-specific expression were defined.
Figure 3 compares random genes and the populations of prospective and approved anti-cancer targets selected by the criteria of tissue-specific expression, see Methods and more detailed presentation in Supporting materials (Text S1, pages 29 46).
We have identified 14 putative SFPs by bioinformatics analysis and found that four of them met the criteria of tissue-specific expression patterns, which led to them being considered as putative SFP.
These cases may not reach strict definitions of self-injurious behaviour that involve tissue injury, but it is important to acknowledge that the criterion for tissue injury for definition of self-injury is somewhat arbitrary.
We review the evolution of microarray platforms used for such studies in order to meet the criteria of complex tissue engineering biological environments.
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