The criteria of differential expression were that a difference of two microRNA is greater than two-fold.
On the other hand a 0 indicates that the probability and/or the fold-change criteria of differential expression are not met.
They identified 197 transcripts that met their criteria of differential expression for both adipocytes and SVF from SC WAT vs. EP WAT; the vast majority of the reported genes were altered less than 3-fold [ 19].
Genes were selected according to the criteria of differential P-values ≤0.05 compared to control (untreated cells or empty vector-transfected cells) and average signals >25 in treated cells.
When a criterion of a differential expression of at least 2.5 fold for assumed biological relevance was applied, IL4, TLR9, TGFB, HMGB1, MIF, NKAP, FAS, and BID were filtered out.
Using a conditional false-discovery rate (cFDR) of 0.01 (permissive criterion for differential expression), EBarrays [ 18] detects over 2204 differentially expressed genes in D. dadantii (48.5%), and 599 in P. atrosepticum (13.4%).
Cell line-specific filtering was done separately for each cell line, following the criteria of a differential expression of at least 2-fold in a minimum of one time point.
Indeed, we identified a group of 16 additional proteins which just missed the cutoff of our criteria for differential expression and which may yet qualify after inclusion of additional samples (Table 3).
Type, frequency and diurnal changes of cough may be criteria for differential diagnosis, therapeutic efficacy, and a gauge for the progression of chronic disease.
A total of 78 probe sets meet these criteria for differential expression in at least one of the harvest times.
