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Based on this criterion, animals injected repeatedly with 2.5 mg/kg MDMA did not express significant change (on experimental days 7, 13, and 38) compared to experimental day 2 in HA (Fig. 3) or TD (data not shown) but showed a significantly increased response in the NOS index on experimental day 13 and experimental day 38 (Fig. 4).
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A positive response for periorbital testing was characterized by the following criteria: animal vigorously stroked its face with the forepaw, head withdrawal from the stimulus, or head shaking.
After analysis of the full text of the obtained manuscripts, articles that did not meet the original criteria (animal leptospirosis in Rio de Janeiro, Brazil) of the review were removed.
Homogeneity in GBW is an important criterion because animals with too low weights at weaning are not desired and animals with very large weights tend to have poorer performances in the subsequent period of food restriction (dry season).
After reaching the extinction criterion, all animals were subjected to a cocaine prime.
On the basis of this criterion, three animals exhibited insufficient lesions and therefore were removed from subsequent analyses.
An escape latency of 10 sec was chosen as the criterion that animals had learned the task, based on previous studies using rats of comparable age in a similar size pool (Jett et al. 1997, 2001; Markwiese et al. 1998; Rudy et al. 1987).
Based on this criterion, one animal per group was excluded from further selection.
Using these criteria seven animals were removed from the biomarker study after injury because of either insufficient or excessive severity.
However, it is often difficult to obtain adequate amounts of information to determine behavioral criteria for animals that spend much of their time out of sight of a human observer, for example in the open ocean.
The exclusion criteria for animals were pre-established.
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