TRIP6, initially identified as a Thyroid Receptor Interacting Protein, met the criterion of binding specifically the N-terminus of POT1 by yeast two-hybrid analysis, and can be detected in a complex with POT1 and other shelterin components in human cells.
The criteria of its binding site are the presence of 7-nt downstream and 3- to 5-nt upstream sequences that are complementary to the acceptor-stem and anticodon-stem domain sequences, respectively, of the 5'-half-tRNAGlu, and a T-arm-like structure in between.
This could be partially the case in the coregulations established by IHF, FNR and NarL (these hubs shared 8 coregulated operons), and only under a permissive criterion (inheritance of binding sites imposes more strict constrains to the location of homolog genes in their respective operons [36]).
Using an ELISA absorbance of greater than 0.2 as the criterion for binding, 99% of the bacterial clones tested secreted fusion phages that specifically recognised CMV.
To achieve this end, we used the following criteria: a) high efficacy of binding of drug to the Ebola virus glycoprotein GP1; b) low toxicity and accessibility (nonprescription drugs); and c) low cost.
For comparing with other binding site prediction algorithms, two widespread adopted datasets from LIGSITEcsc [29] were used for testing our algorithm with the same criteria of evaluating the accuracy of binding site prediction.
Also in order to control false positive rate of prediction, we concentrated on the methods that use stringent seed-matching criteria and consider the evolutionary conservation of binding sites [34].
We therefore investigated the Sec61α/γ sub-complex as an alternative system, using the criteria of stable co-association, cotransin binding, and RNC targeting as three independent indicators of correct folding and partial functionality.
Similarly, in the case of amylase (PDB: 1byb/1bya, RMSD = 3.10 Å), the binding sites of two of the four sugar residues were correctly predicted with the criterion of 'partially correct binding site' (Fig. 5C).
One criterion is that a Siglec protein is capable of binding sialylated glycans.
