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This template is common to all CAPs investigated and was used as an anchoring criterion of alignment and refinement thorough the rest of this study.
For each iteration, the criterion of alignment length Taln was incremented by 30 bp if Taln was less than or equal to 300 bp.
An additional drawback is that using only the single center of mass point in each projection removes the use of any local structure of the projections as criteria for alignment.
To ensure that all results are robust to inclusion criteria, we also used the less restrictive criteria of 50% alignment overlap and 40% amino acid sequence identity.
Manual annotation under stringent criteria of sequence alignment with other dimorphic fungi gene sets allowed us to ascribe a putative biological function to many of those genes.
Parsing of BLASTx results was done with the criteria of an alignment length of at least 34 amino acids [ 31] and a minimum of 35% identity, as suggested by the Gene Ontology Consortium (http://www.geneontology.org/GO.annotation.SOP.shtml).org/GO.annotation.SOP.shtml
Both the libraries were annotated by the blastX program against the Uniprot database, at criteria of 50% alignment length, and evalue of 1 × 10-10 (Additional file 13; worksheet 1 and 2).
Short read contigs in the size range of 50-100 bp were mapped using Megablast [ 21] and short read contigs of 100 bp and longer were mapped using BlastZ [ 22] with contig alignment criteria of at least 80% alignment and at least 60% sequence identity.
We have applied stringent criteria for generation of alignment and also have incorporated paired end reads for identification of genuine SNVs from noise.
In addition, we used the column score where the percentage of correct columns in an alignment is the criterion for alignment quality.
Whole-genome comparisons, particularly between distantly related genomes such as human and fish, fail to identify and align all the orthologous sequences due to the stringent criterion of local alignment algorithms.
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