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Criteria for quantification: 1. hits were only accepted at a threshold of two peptides e≤0.1; 2. spectral count of at least 3 in at least 4 of the samples of one sample set was required for acceptance.
We found indications that the levels of a JH-degrading enzyme (JH epoxide hydroxylase) increase with time in diapause (data not shown because this protein barely missed our criteria for quantification).
In addition, when selection criteria for quantification were made less stringent, two additional enzymes that are putatively involved in glycerol metabolism (phosphoglucomutase and triose-phosphate isomerase) were found at higher levels during at least one of the diapausing stages (data not shown).
Qualitative inter-observer agreement for the anatomical structures which met the criteria for quantification was also substantial (PABAK = 0.76).
FASSS meets essential validation criteria for quantification of a common structural abnormality in clinical trials of axial spondyloarthritis.
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In this study, 242 metabolites fulfilled quality criteria for relative quantification, and absolute quantification was performed for an additional 14 metabolites.
A set of criteria for linguistic quantification was proposed in the previous literature.
Our assay, which included PCA extraction, online desalting, separation of the high-energy phosphates on a C18 reversed-phase column using a methanol/dibutylammonium formate gradient, and detection of negative ions in the single ion mode, met all predefined acceptance criteria for the quantification of AMP, ADP, ATP, CDP, CTP, FAD, GDP, GTP, UDP, and UTP.
The effect of metal-quenching on CAS excitation and emission conditions (λex=300 nm; λem=407 nm) was used as criterion for analyte quantification in presence of sodium cholate bile salt (NaC).
Although this stringent threshold lowers the number of genes for which differential expression can be performed, we believe that this criterion is sufficient for quantification and analysis of highly expressed genes with a corresponding reduction in the number of Type I errors due to lowly expressed genes [ 35- 37].
We set criteria for acceptance of quantification results based on the Cq of the internal control falling within a 1 log range; outside of this range, quantitative results were not accepted, owing to the potential for partial PCR inhibition leading to inaccuracy of measurement.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com