In the table, detailed information, including the IOI, weight values of criteria, cell numbers of classes (S1 S5), and changes in cell numbers between two classes with the change of IOI value are listed.
Gating was done to exclude by forward and side scatter criteria cell debris and cell clumps.
The cell cycle stages at which the TgAP2yfp fusion proteins were expressed was determined by anti-IMC1 (daughters), anti-YFP (AP2s), and DAPI (genomic DNA) co-staining, employing criteria (cell size and marker expression) we previously established to pinpoint cell cycle arrest in a large collection of conditional mutants [38].
Based upon these criteria, cell fusion was quantified in each animal by counting the percentage of crypt units (crypt/villus or crypt/cuff in the small intestine or colon, respectively) that contained GFP-expressing epithelial cells in a total of 1500 crypt units.
Representative large sections of each tumour were evaluated for the following morphologic criteria: cell type, cellularity, mitotic rate, presence of tumour necrosis, and of giant cells [ 26].
Nonetheless, with different criteria, cell death can be classified into different subroutines and different subroutines of cell death own a distinct molecular mechanism and morphological characters and perform different roles in regulating the fate of cells [ 4].
For example, to prompt for the city, click the Criteria cell for the city field, and type [Which city?].
SDS gel electrophoresis was executed with precast gels in a Criterion-Cell (BioRad).
The following precast gels were used for the expression studies: 4 20% Criterion precast gradient gel (BioRad #354-0032) in a Criterion-Cell (BioRad).
