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Monte Carlo simulations are carried out for topologies resembling both tubular and lamellar cristae, for a range of physiologically viable crista sizes and densities.
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The edge scores for the first and last sections of each mitochondrion were determined according to established criteria: 0.25 for light gray without visible cristae, 0.50 for medium gray without cristae, 0.75 for black without cristae, and 1 when mitochondrial cristae were visible62.
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In all mitochondria, the cristae account for most of the inner membrane surface, highlighting their importance for cellular physiology.
Number of cristae junctions for the lamellar cristae is between 1 and 6.
Upon electron microscopy of aged Pink1−/− brain, the loss of cristae reported for Drosophila melanogaster could not be demonstrated (Fig. 7G H).
Thus, enforced fusion is not sufficient to correct the mitochondrial apoptotic phenotype of HD models, further highlighting the importance of Opa1-dependent cristae shape for their increased apoptosis.
In both species, stereological analysis confirms that oocyte mitochondrial cristae account for a much lower proportion of total mitochondrial volume than in other tissues (fig. 4 d and h for Drosophila and zebrafish, respectively).
This finding is consistent with previous TEM analyses on other animals in which mitochondria from fully grown oocytes typically show a dense matrix traversed by numerous cristae (see for example Actinia fragacea [ 32]).
Fig. 4A should be compared to Fig. 5A, since in both cases we change the main geometric parameter: L for the tubular cristae and Rlam for the lamellar ones.
It has been suggested that there may be a threshold for the density of cristae junctions required for the maintenance of nucleoid distribution (Itoh et al, 2013).
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