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Since neural crest generation has already been initiated at neural fold stages, these results may reflect mechanisms of regeneration and maintenance of inducing capacity rather than the mechanism by which neural crest cells are initially induced.
This period of development corresponds to the timing of neural crest generation and initial forebrain patterning.
In vertebrates, they are crucial for embryonic patterning as well as neural tube formation, neural crest generation, and mesodermal segmentation.
Although the phenotypes were too varied to identify the molecular and cellular causes of each phenotype, the cell death that is distributed throughout regions of the neuroepithelium around the time of neural crest generation might explain a majority of the malformations observed here.
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Hopes that the worst unrest in Britain in a generation had crested and begun to fall continued to weigh uneasily against fears that more robust police action might fail to put more than a temporary curb on the disorder.
In our culture system, the generation of neural crest cells was observed during the differentiation process.
In addition, our results provide evidence that attenuation of Wnt signaling in caudal border cells blocks the generation of neural crest cells and promotes the generation of olfactory and lens placodal cells normally generated from rostral border cells.
Both BMP and Wnt signals have been implicated in the generation of neural crest cells at the neural fold stage when neural plate border cells have started to express neural crest markers [20], [21].
Thus at stage 4, when Wnt signaling is suppressed in prospective caudal neural plate border cells, the generation of neural crest cells is blocked and cells acquire olfactory and lens placodal character characteristic of cells derived from the rostral border.
We tested therefore whether by stage 6 the generation of neural crest cells also has become independent of Wnt activity, by exposing stage 6 CB explants isolated at the midbrain-hindbrain level to mFrz8CRD for 18 20 hr.
At this stage, BMP signaling is ongoing in both rostral and caudal border cells [6], [7], and our results provide evidence that exposure of rostral border cells to Wnt signals induces the generation of neural crest cells at the expense of olfactory and lens placodal cells.
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