I didn't have an assistant, so we all mucked in, chucking smoke bombs to make it seem like a warzone and creating wounds with fake blood.
They just swoop down when a whale surfaces to breathe, then use their beaks to slash open its rubbery skin, creating wounds up to 4 inches deep and 5 feet long.
But in political terms, the 187 campaign felt to many of the state's emerging Latino voters like an attack, creating wounds that have yet to heal, says Lisa Garcia-Bedolla, a political scientist at the University of California, Berkeley.
The rats were anaesthetised by intramuscular injection of ketamine and xylazine prior to creating wounds.
The cell monolayer was scratched using a fine plastic pipette, creating wounds of approximate 250 μm in width.
Upon confluence a high field current with frequency 48 kHz and amplitude 5 was applied for 10 seconds which killed cells overgrowing the electrode, creating "wounds" in the wells devoid of cells.
Our CIA technique overcomes these problems, at least in part, by creating wounds with a soft silicon tip in place of using blunt trauma, which leaves both ECM and cells intact (see Additional files 1 and 2).
Furthermore, since a standard tip and automatic drill is used for creating "wounds", the original size, shape, and spacing of replicates was found to be highly reproducible, and the assay was easily quantified.
Two 4-0 vicryl threads are applied to each side of the created wound, followed by suturing to the mucosa on the nasal side.
Treatment with LLL12 resulted in a decreased ability for cells to migrate and heal the created wound (Figure 5).
