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This design allows cloning of the DNA fragment of interest efficiently via BpiI - removing the BpiI recognition sites and lacZα in the process - but provides the possibility to release the cloned fragment with BsaI creating the identical fusion sites it was cloned in.
On both sides of the lacZα fragment two different type IIS recognition sequences - here BsaI and BpiI - are positioned in inverse orientation relative to each other, but creating the identical fusion site as exemplified by plasmid pL0-P in figure 3.
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The resultant growth disturbance created the identical deformity pattern of distal femoral procurvatum and shortening (Fig. 1a, b).
His executive order creating the mandate committee used identical language to the order forming the Medicaid committee, charging the group with drafting proposals "for consideration in the budget process".
Imagine a large number of copies of the system being created in the identical starting condition at time 0, with the same initial microstate and the same protein components present in the surrounding solution at the same concentrations.
Last summer 631,064 visitors trekked through "Big Bambu: You Can't, You Don't, and You Won't Stop," the huge sculpture created by the identical-twin artists Mike and Doug Starn.
But in animation, there are no limits, and there's no other art form that can re-create the imagination purely, just identical".
Separate versions of the interviews suitable for children were created; the conceptual content was identical to that of the adult version although minor, but important changes, in wording (and/or sequence) were required.
This Act prohibits procedures undertaken for the purpose of creating genetically identical human individuals and prohibits the creation of embryos for research.
This expanded the coastal plain, creating habitats identical to those currently inhabited by species of Pteronotropis and is herein hypothesized to have provided for a northern expansion of populations.
See also: Ghost, Shaun Of The Dead, Property Ladder Wilmut's Cloned Sheep = CSI Cloning is the biological process of creating an identical copy of something's DNA cells.
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