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L1 gene was released from pBSK after double-digestion with XhoI and NotI and employed for creating the expression vectors.
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No one has evidently disputed his claim that he created the expression sci-fi.
Inserts cloned into the entry clones were subsequently recombined into the destination vectors via an LR reaction to create the expression constructs.
Invitrogen gateway cloning was used to create the expression constructs from pEnter.
BCG created the expression construct and created the transgenic plants from which the hairy-root cultures were derived and participated in all of the above activities.
Furthermore, the resulting gene expression clusters can be immediately linked to the specific transcription factors whose action created the expression profile.
MW created the expression constructs, carried out transient expression experiments, helped with animal experiments, did serum analysis and drafted the manuscript.
Entry vectors were assembled together with the pDestTol2pA2 by LR reaction to create the expression vector pDestTol2-Kdrl-Ubiad1-2A-mCherry-pA.
This vector was subsequently transferred into the pcDNA-DEST47 destination vector to create the expression clone pEXP-CLU which allows for constitutive gene expression under the control of a CMV promoter.
The purified PCR fragment and the pAID4T vector were ligated to create the expression vector, in which the ActRIIB ECD was fused in-frame to the human IgG1 Fc domain.
To create the expression constructs, the cDNA inserts of the entry clones were shuttled into the pEF-DEST51 destination vector (Invitrogen; 12285-011) using LR-clonase recombination (Invitrogen; 11791-020) according to the manufacturer's instructions.
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