Sentence examples for creating constructs from inspiring English sources

We have investigated the role of the human topoII C-terminal domain by creating constructs encoding C-terminally truncated recombinant topoIIα and β and topoIIα+β-tail and topoIIβ+α-tail chimeric proteins.

To this end we removed the two 5'-splice sites, the potential poly-A site or all from the human sequence creating constructs pTM167, pTM165 and pTM164 respectively.

Isoforms 1-3 conoaining no stop codons were then cloned into a version of pcDNA3.1 containing the HA epitope between restriction sites XbaI and ApaIon the pcDNA3.1 vector creating constructs Iso1-HA, Iso2-HA, and Iso3-HA.

In creating constructed novel ecosystems within the OCBIL landscapes of Western Australia, the techniques within a baroque toolbox succour the local by embracing and enhancing intrinsic ephemera and site specificities.

The PCR fragment was sub-cloned into pGEMTeasy vector (Promega), and ligated to pGL3-basic (SmaI site), creating construct 375a, or to TK-luc.

The method was successfully optimized and applied to create constructs containing promoter, RBS and/or mutant enzyme libraries.

We created constructs encoding the full-length S1R with APEX2 attached at either the N- or C-terminus of the receptor (Fig. 1B).

Organ-on-a-chip engineering employs microfabrication of living tissues within microscale fluid channels to create constructs that closely mimic human organs.

Shawn Ashmore as Bobby Drake / Iceman A young mutant, Iceman can create constructs of ice or blasts of cold.

To identify amino acids in SMN that are important for calpain cleavage, we created constructs containing internal deletions in EGFP-SMN and tested their susceptibility to calpain cleavage in cell-free assays.

We therefore created constructs where we mutated either the FOXL2 or the SMAD3 site in the composite element, and a construct in which the whole element was removed.