Sentence examples for creating a reporter from inspiring English sources

Furthermore, our toolkit provides a novel recombineering cassette which inserts a SL2-spliced intercistronic region between the gene of interest and the fluorescent protein, thus creating a reporter controlled by all 5' and 3' cis-acting regulatory elements of the examined gene without the direct translational fusion between the two.

In order to monitor Cre recombinant expression in vivo, it is important to create a reporter strain.

The aforementioned results demonstrate that we have successfully created a reporter gene tool to monitor the boundary activity of the IR sequence.

We have employed computational design for the semi-rational engineering of human 2′-deoxycytidine kinase to create a reporter gene with selectivity for l-nucleosides including l-thymidine and 1-(2′-fluoro-5-methyl-β l-arabinofuranosyl) uracil.

The reaction was incubated at 25° for 5 min, 50° for 1 hr then 70° for 15 min. The resulting cDNA was analyzed in 15-µL quantitative polymerase chain reactions in three technical replicates using primers listed in Table 2. To study boundary activity, we created a reporter system to assess boundary activities via simple plate assays.

The entire 3′UTR of PIAS3 was cloned into the pcDNA-GL3 control vector, creating a luciferase reporter gene containing the seed match for miR-18a.

Each promoter also was subcloned into pGRB2.3 using SacI/ XbaI or SacI/ SpeI restriction digestions, positioning the promoter upstream of the MCS and GFP open reading frame, creating a GFP reporter plasmid.

A similar readout can be obtained by creating a "dead" luciferase reporter plasmid [ 26 ].

With their client, e-tractions Inc., a small Web entertainment company based in Bedford, Mass., the Sterling Hager people concluded that they could attract the media by creating a venue for reporters to whack flacks.

Earlier this year, The Associated Press announced that it was creating a team of reporters to cover issues surrounding marijuana legalization.

For this work, the Renilla luciferase gene (SRUC3) was recloned from the plasmid pBluescript/SRUC3 [ 24] to replace the EGFP gene in pEGFP-N1, creating pSRUC3-N1, a reporter plasmid with expression of SRUC3 under the control of the CMV promoter.