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Passage of the created sequence into an accumulated generating operation (AGO).
We created sequence logos of the 20 most N-terminal residues (Figure 7A) and aa frequency distribution plots over the entire predicted cTP (Figure 7B) for the 916 annotated nuclear-encoded plastid proteins (excluding outer envelope proteins).
We also created sequence logos of the motif regions observed in each genome.
We created sequence logos of 11 residues using the WebLogo application [ 32], which included known and potential phosphorylated sites in the central positions of these conserved phosphorylation motifs.
We have created sequence logos for observing amino acid distribution of consensus positive sites, non-consensus positive sites, consensus negative sites, and non-consensus negative sites.
To illustrate the PAS sequence landscape, we created sequence logos for the flanking regions, which visually showed that the conserved motifs were found in the region (−30,+30) nt, WebLogo (Crooks et al., 2004), Figure 4c.
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Aptamers are generally selected from the pools containing randomly created sequences through an in vitro systematic evolution of ligands by exponential enrichment (SELEX) [7].
Additionally, major sequence data providers such as GenBank (27), EMBL (28) and DDBJ (29) do not maintain a list of artificially created sequences.
It is highly implausible that these newly created sequences have not played a role in the evolution of maize genes and of maize.
Five genomic libraries were created, sequenced, and MAQ-assembled using both haploid and diploid parameters as described above (also see Table S1).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com