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Based on our docking results, we created, expressed and purified the chimeric S194G-1 mutant of 3β-HSD1.
In the present study, three mutants of COS BTPC (RcPPC4) were created, expressed, and purified: (i) S425D, a phosphomimetic substitution; (ii) S425A, a mutagenesis control; and (iii) P426A, the substitution of the adjacent proline residue.
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Then, for each one of the dynamic CE, a new design task should be created, expressing the need to monitor it.
Mice were also created expressing SR-BI exclusively in endothelium and liver.
To test this, transgenic maize (Zea Mays L. Hi-II hybrid) lines were created expressing soybean vspB from a maize ubiquitin Ubi-1 promoter.
A complementary cell line was created, expressing a DD-GFP-mCherry fusion protein that was tagged with a nuclear export sequence.
Biotechnological approaches mimic this principle, through the engineering of gene fusions to create expressed proteins, ones combining formerly separate components into a single polypeptide chain.
In general, he holds that verbs such as "create" express nothing in the creator himself but rather something in things.
Founder Robert Scott and his team have created a space for like-minded individuals to create, express and share.
Those great artists notwithstanding, the settlements' perspective on the arts began with an understanding that the impulse to create, express and connect is universal, that we all are culture-makers, and that art is highly social, moral and cognitive.
Besides doubling parking fines recently, the mayor has also turned 10 streets in Midtown into no-turn zones for most of the day to ease crosstown traffic, and created express lanes in Lower Manhattan for bus use.
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