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To permit rapid identification of the K-Ras codon 117 mutation, a PCR RFLP assay was designed where the presence of the mutation created a recognition site for the restriction enzyme Bsr S1.
Similarly, a PCR-RFLP assay was designed to identify the K-Ras codon 146 mutation, where the presence of the mutation created a recognition site for the restriction enzyme Mse I.
To see whether we could find a sharper tool in distinguishing between idiopathic and malignancy-associated MN, we created a recognition category score based on the four distinct categories of staining pattern that are possible, Supplementary Figure S2.
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Ms. Claggett said she was recently asked to create a recognition program commemorating the anniversaries of the company's 400 franchisees.
Meanwhile, the government - as required by royal charter - is in the process of creating a recognition body in accordance with the recommendation in the Leveson report.
It will also administer targeted training grants, develop compliance assistance tools, forge partnerships, and create a recognition program to highlight successful ergonomics injury reduction efforts.
Plk1 phosphorylation initiates the destabilization of Wee1 by creating a recognition sequence for the F-box protein β-TrCP that cooperates with the SCF ubiquitin-ligase [4].
A single T (A1 allele) to C (A2 allele) nucleotide substitution in the 5'-untranslated region of CYP17 creates a recognition site for the MspA1 restriction enzyme.
Any amplicon with a polymorphism in dbSNP (build 129) that either could remove or create a recognition site for any of the five MSREs was removed.
This creates a recognition motif for an E3-ubiquitin-ligase complex that contains β−transducin-repeat-containing protein (β-TRCP), which tags the protein for proteasomal degradation.
Interestingly, Bora degradation appears to involve a negative feedback loop: PLK1 phosphorylation of Bora creates a recognition site for the E3 ubiquitin ligase SCF-betaTrCP [ 46].
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