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Additional file 1 contains the information used to create the linkage map.
These data could then still be used to create the linkage maps.
Most participants were not concerned about the initial necessary use of identifiable data to create the linkage key.
Transcriptome sequencing and high-throughput SNP genotyping were performed in both species to create the linkage maps.
The TWOPOINT function was used with a LOD score threshold of 50.0 in order to create the linkage groups.
With regard to laboratory tests, we selected plasma glucose and HbA1c levels to create the linkage with clinical and administrative databases.
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MR conducted the salinity tolerance trials, performed the genome scans and created the linkage maps.
Resulting loci that were differentially fixed between the grandparents, in Hardy-Weinberg equilibrium, and successfully genotyped in 100 or more F2 individuals were chosen for creating the linkage map.
The overall consent preferences for all scenarios are summarized in Table 3.> Participants who supported the 'no consent' option did not view the initial disclosure of personally identifying information for the purpose of creating the linkage key as a concern but some did indicate that the data linkage organization would need to be trustworthy.
The swing to the consent option and the justifications provided in Scenario 4 indicated great unease when an independent organization is not involved in creating the linkage key and therefore researchers need to deal with this aspect before they can analyze de-identified data.
Genotypic data generated using the RAD-seq and Illumina GoldenGate were used to create the genetic linkage map of CPR-01.
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