Sentence examples for create marker from inspiring English sources

Although the molecular toolkit of F. graminearum is well developed compared to other fungi, Cas9-mediated engineering offers a number of potential benefits, such as the ability to create marker free mutants in this species.

It has also been demonstrated that PiggyBac transposons can be used to create marker free induced pluripotency stem cells [ 13].

The most polymorphic loci can be added to other SSR loci to create marker sets for genetic diversity analysis in P. dactylifera and other species.

We need to create markers and milestones that capture incremental changes in financial and personal well-being and in the lives of children.

In this study, we used two improved versions of the piggyBac transposase (PBase) to create marker-free transgenic strains of the unicellular green alga Coccomyxa sp. strain KJ as follows: Uracil-auxotrophic (Ura−) mutants of strain KJ defective in the gene for uridine monophosphate synthase (KJUMPS) were isolated on agar plates containing 5-fluoroorotic acid.

– The ability to create markers and info windows with less code.

Following its report, the Equal Justice Initiative said it hoped to create markers and memorials throughout the South denoting the sites of notorious lynchings as well as slave markets and other dark, unacknowledged aspects of American history, in order "to force people to reckon with the narrative through-line of the country's vicious racial history," according to The New York Times.

To create marker-less non-polar deletion mutants of pilP, NgonM_03101, pilQ and pilE, PCR fragments of the flanking regions of the respective genes were annealed using the splicing by overlapping extension PCR (SOE-PCR) [63] method.

Based on this finding, we developed a systematic and scalable method to create marker-free mutations, insertions, and deletions at any locus.

In summary, we have found that short homology arms stimulate HDR at high-enough efficiency in the C. elegans germline to create marker-free, gene-sized edits in a single step.

Any user can create markers with faculty markers being visually distinct from student markers.